mtnD [2017-8-11 16:0:3]

1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase

locus: BSU13620

pI: 4.41

mw: 20.68 kDa

protein length: 178 aa

gene length: 534 bp

function: methionine salvage

product: 1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase

essential: no

synonyms: ykrZ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 1,429,584 → 1,430,120

The protein

Catalyzed reaction/ biological activity

• 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one O₂ = 3-(methylthio)propanoate formate CO (according to Swiss-Prot)

Protein family

• acireductone dioxygenase (ARD) family (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]-[gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]-[gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]-[gene|C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9|mtnD]
  description: [Pubmed|12022921]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]

Biological materials

Mutant

• MGNA-A786 (ykrZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/786 NBRP B. subtilis, Japan]

References

• 12022921,11914366,12107147,15102328,12787499,18039762,15378759